Mutation detection using a novel plant endonuclease

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Biology 102

Exam IV  December, 2004

1.Which of the following does not directly participate in translation?

a. mRNA

b. ribosomes

c. signal peptides

d. amino-acyl tRNA molecules

e. polysomes

2. The anticodon of a particular tRNA molecule is

a. changeable, depending on the particular amino acid that is attached to the tRNA

b. catylitic, making the tRNA into a ribozyme

c. complementary to its binding site on the P site of the large subunit of the ribosome

d. complementary to the complementary mRNA codon

3. In the lac operon, which of the following does not have an impact on transcription?

a. the concentration of cAMP

b. the concentration of lactose

c. the binding of CAP

d. attachment of the poly-A tail

4. During post-transcriptional processing in a eukaryotic cell, which of the following does not occur?

a. attachment of the 5’cap

b. removal of intron sequences in the spliceosome

c. attachment of the 3’ polyA tail

d. attachment of the polysome to the endoplasmic reticulum

e. all of the above occur during post-transcriptional processing

5. If one strand of a DNA double helix has the sequence of bases 5'-ATTGCA-3', the complementary strand would have the sequence:

a) 3'-UAACGU-5'   b) 3'-TAACGT-5'

c) 3'-TUUCGT-5'   d) 5'-TAACGT-3'

e) 5'-TUUCGT-3'

6. If a portion of a genic sense strand of a part of a DNA double helix is 5’ATTGCA-3’, the corresponding antisense strand is

a) 3'-UAACGU-5'   b) 3'-TAACGT-5'

c) 3'-TUUCGT-5'   d) 5'-TAACGT-3'

e) 5'-TUUCGT-3'

7. If the antisense strand of a part of a gene reads 5’CATTCA, and this is complementary to two complete codons, the amino acids encoded are:

(you look up the table and fill this in.)

8. A complete, infective HIV viral particle contains two complete genomes and two copies of this enzyme:

a) DNA polymerase

b) amino acyl tRNA synthase

c) Reverse transcriptase

d) DNA-dependent RNA polymerase

e) Sequenase

9. Viral genomes consist of:

a) single stranded DNA

b) double stranded DNA

c) single stranded RNA

d) double stranded RNA

e) all of the above

10. Which of the following processes would never contribute to genetic variation within a bacterial population?

a) transformation

b) transduction

c) conjugation

d) specialized transduction

e) meiosis

11. What is the central dogma of molecular genetics?

a) life begets life

b) spontaneous generation

c) DNA->RNA->protein

d) Protein->RNA->DNA

e) Enzymes are the basis of life

12. Transposition differs from other mechanisms of genetic recombination because it

a) occurs only in bacteria

b) moves genes between homologous sequences during meiosis

c) moves genes to new loci in the genome

d) occurs only in eukaryotes

13. During conjugarion between an Hfr cell and an F- cell,

a) the chromosome of the F- cell is always completely displaced by the chromosome of the Hfr cell

b) DNA from the F- cell transfers to the Hfr cell

c) Genes from the Hfr cell may replace genes of the F- cell by recombination

d) Sexual gene transfer may be disrupted by a Waring blender

e) c and d

14.  What enzyme is responsible for the ‘blue’ color of cells transformed with non-recombinant plasmids (those developed by J. Messing)?

a) Alpha amylase

b) beta amylase

c) amino acyl tRNAsynthase

d) beta-galactosidase

e) alpha-glucosidase

15. In a nucleosome, the DNA is wrapped around

a) ribosomes

b) histones

c) the nucleolus

d) transposons

e) RNA polymerase

16. Rearrangement of DNA segments is known to occur in genes coding for

a) ribosomal RNA

b). most eukaryotic proteins

c) antibodies

d) histone proteins

e) alpha amylase

17. The amount of protein made from a given mature mRNA molecule depends partly on

a) the degree of DNA methylation

b) the rate at which the mRNA is degraded

c) the number of introns in the mature mRNA

d) the types of ribosomes available

e) the amount of DNA polymerase present in the cytoplasm

18. The most efficient transforming bacterium known is:

a) E. coli

b) S. typhimurium

c) P. syringae

d) A. tumefaciens

e) H. vulgare

19. Golden rice produces greater than normal amounts of

a) alpha amylase

b) beta glucanase

c) beta-carotene

d) Vitamin K

e) keratogenic particles

20. One of the currently utilized human gene vector systems utilizes

a) Barley stripe mosaic virus

b) hantavirus

c) adenovirus

d) smallpox virus

e) septovirus

21. Embryonic stem cells are regarded as a potentially key therapeutic agent because of their:

a) omniscience

b) immunoresponsiveness

c) disease resistance

d) totipotency

e) mutagenicity

22. Which has the largest genome and the lowest number of genes per million basepairs?

a. H. influenzae (the bacterial cause of a version of flu)

b. S. cerevisiae ( the yeast that produces beer and bread)

c. A thaliana (a small and insignificant plant that would be a weed if our crops were more feeble)

d. D melanogaster (fruit fly)

e. H. sapiens

23. Which of the following was key to the development of recombinant DNA techniques?

a. DNA sequence analysis

b. the one-gene one-enzyme concept

c. Taq polymerase

d. Restriction endonucleases

e. Alpha amylase

24. In recombinant DNA methods, the term vector refers to

a) an enzyme that cuts and inserts one DNA molecule into another

b) T4 ligase

c) Taq polymerase

d) a plasmid that contains both restriction sites and an origin of replication

e) the sticky end of a DNA molecule

25. Taq polymerase

a) synthesizes proteins using antibodies as templates

b) synthesizes RNA using primers as starting points

c) synthesizes DNA using sequence-specific primers as starting points

d) synthesizes carbohydrates using oligosaccharides as templates

e) synthesizes lipids using cholesterol as a starting point